✦ LIBER ✦

Molecular Analysis of the Cryptococcus neoformans ADE2 Gene, a Selectable Marker for Transformation and Gene Disruption

✍ Scribed by Sunil Sudarshan; Robert C Davidson; Joseph Heitman; J.Andrew Alspaugh

Book ID: 115609025
Publisher: Elsevier Science
Year: 1999
Tongue: English
Weight: 688 KB
Volume: 27
Category: Article
ISSN: 1087-1845
DOI: 10.1006/fgbi.1999.1126

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Characterization of the L41 gene in Cryp

Characterization of the L41 gene in Cryptococcus neoformans: its application as a selectable transformation marker for cycloheximide resistance

✍ A. Varma; K. J. Kwon-Chung 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 174 KB 👁 2 views

A transformation system using resistance to the antibiotic cycloheximide as a dominant selectable marker was developed for the pathogenic yeast Cryptococcus neoformans. A 3.5 kb DNA fragment containing a gene encoding the ribosomal protein L41 was cloned from a wild-type strain of C. neoformans whic

Co-transformation using a negative selec

Co-transformation using a negative selectable marker gene for the production of selectable marker gene-free transgenic plants

✍ Jeongmoo Park; Young Koung Lee; Bong Kyoon Kang; Won Il Chung 📂 Article 📅 2004 🏛 Springer 🌐 English ⚖ 257 KB

A novel episomal shuttle vector for tran

A novel episomal shuttle vector for transformation of Cryptococcus neoformans with the ccdB gene as a positive selection marker in bacteria

✍ Philippe Mondon; Yun C Chang; Ashok Varma; Kyung J Kwon-Chung 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 335 KB

A novel episomal shuttle vector for tran

A novel episomal shuttle vector for transformation of Cryptococcus neoformans with the ccdB gene as a positive selection marker in bacteria

✍ Philippe Mondon; Yun C Chang; Ashok Varma; Kyung J Kwon-Chung 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 337 KB

The dominantHc.SdhRcarboxin-resistance g

The dominantHc.SdhRcarboxin-resistance gene of the ectomycorrhizal fungusHebeloma cylindrosporumas a selectable marker for transformation

✍ Chrisse Ngari; Jean-Philippe Combier; Jeanne Doré; Roland Marmeisse; Gilles Gay; 📂 Article 📅 2009 🏛 Springer-Verlag 🌐 English ⚖ 436 KB

Genetic engineering of Schizosaccharomyc

Genetic engineering of Schizosaccharomyces pombe: A system for gene disruption and replacement using the ura4 gene as a selectable marker

✍ Grimm, Christian ;Kohli, Jürg ;Murray, Johanne ;Maundrell, Kinsey 📂 Article 📅 1988 🏛 Springer 🌐 English ⚖ 702 KB

A system is described for gene disruption and replacement in Schizosaccharomyces pombe based on the homologous selectable marker, ura4, the structural gene for orotidine-5'-phosphate decarboxylase. The presence of a single copy of the wild-type gene can rescue a ura4 auxotrophic mutant. Furthermore,