✦ LIBER ✦

Glycosylation analysis of glycoproteins and proteoglycans using capillary electrophoresis-mass spectrometry strategies

✍ Scribed by Sabine Amon; Alina D. Zamfir; Andreas Rizzi

Book ID: 102832709
Publisher: John Wiley and Sons
Year: 2008
Tongue: English
Weight: 671 KB
Volume: 29
Category: Article
ISSN: 0173-0835
DOI: 10.1002/elps.200800105

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

This review highlights recent developments in glycosylation analysis by modern MS in combination with CE based preseparation. Focused on CE‐MS strategies aimed for glycotyping, the review addresses the detailed glycoform analysis of glycoproteins, glycopeptides, and proteoglycans. Glycoform analysis in the context of modern glycoproteomics is briefly addressed, as well. CZE, CIEF, and frontal analysis CE approaches hyphenated to high‐resolution multistage MS for the detailed analysis of protein‐linked glycan structures are overviewed in a comprehensive way. Advantages and limitations of various methodological approaches and techniques as well as mass spectrometric instrumentation are discussed in the particular context of glycoanalysis.

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