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Determination of sialic acids released from glycoproteins using capillary zone electrophoresis/electrospray ionization mass spectrometry

✍ Scribed by Karin Ortner; Wolfgang Buchberger


Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
232 KB
Volume
29
Category
Article
ISSN
0173-0835

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A simple and highly sensitive capillary electrophoresis (CE) method for determining the content of N-acetylneuraminic acid (Neu5Ac) in glycoproteins was developed. Neu5Ac was derivatized with 2-aminoacridone (AMAC) by reductive amination, and the AMAC-Neu5Ac adduct could be readily separated from th