Capillary electrophoresis-mass spectrometry using noncovalently coated capillaries for the analysis of biopharmaceuticals
β Scribed by R. Haselberg; V. Brinks; A. Hawe; G. J. de Jong; G. W. Somsen
- Book ID
- 105894147
- Publisher
- Springer
- Year
- 2011
- Tongue
- English
- Weight
- 463 KB
- Volume
- 400
- Category
- Article
- ISSN
- 1618-2650
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## Abstract The usefulness of a noncovalent capillary coating consisting of two layers of oppositely charged polymers for the separation of peptides with capillary electrophoresis (CE) was studied. Capillaries were coated simply by subsequently flushing with solutions of 1% m/v Polybrene and 1% v/v
## Abstract The usefulness of a noncovalent, positively charged capillary coating for the efficient analysis of intact basic proteins with CE was studied. Capillaries were coated by subsequent flushing with solutions of 10% w/v Polybrene (PB), 3% w/v dextran sulfate (DS), and again 10% w/v PB. Coat