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Glutamine synthetase activity in WI-38 cells

✍ Scribed by Dace Viceps; V. J. Cristofalo

Publisher: John Wiley and Sons
Year: 1975
Tongue: English
Weight: 325 KB
Volume: 86
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040860103

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✦ Synopsis

Abstract

In the presence of complete growth media (Eagle's MEM), human diploid WI‐38 cells have a low level of glutamine synthetase activity. The activity could be increased by depriving the cells of exogenous glutamine; addition of hydrocortisone to either glutamine‐deficient or complete medium had no effect on the activity of the enzyme. Cell growth ceased under conditions that enhanced glutamine synthetase activity, and hydrocortisone could not reverse this inhibition.

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