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Transcriptional activity of nuclei from WI-38 cells at various passages

✍ Scribed by Kiyoshi Nose; Hiroshi Okamoto

Publisher: John Wiley and Sons
Year: 1980
Tongue: English
Weight: 263 KB
Volume: 102
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041020108

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✦ Synopsis

Abstract

Transcriptional activity of nuclei from human diploid fibroblast (WI‐38) cells at different passage levels was studied with endogenous RNA polymerase. The rate and extent of the RNA synthesis decreased when the nuclei were were prepared from senescent cells. The decreased activity of RNA synthesis in senescent cell nuclei was demonstrated from both the transcriptional assay and the estimation of the processing activity, i.e., polyadenylation of the transcripts.

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