✦ LIBER ✦

Glutamine regulates glutamine synthetase expression in skeletal muscle cells in culture

✍ Scribed by Bo Feng; Susan K. Shiber; Stephen R. Max

Publisher: John Wiley and Sons
Year: 1990
Tongue: English
Weight: 699 KB
Volume: 145
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041450224

No coin nor oath required. For personal study only.

✦ Synopsis

Metabolite control of glutamine synthetase expression (by glutamine) was studied in L6 skeletal muscle cells. Depletion of glutamine from the culture medium for 24 hours resulted in a 3-4-fold increase in glutamine synthetase activity. This effect was blocked by cycloheximide but not by actinomycin D. Addition of glutamine to L6 cells maintained in glutamine-free medium caused a rapid return of glutamine synthetase activity to the control level. As reported, dexamethasone caused a striking increase in the glutamine synthetase mRNA level. In contrast, neither depletion nor addition of glutamine caused a change in the glutamine synthetase mRNA level. Therefore, regulation of glutamine synthetase by glutamine is exerted at a post-transcriptional level.

📜 SIMILAR VOLUMES

Regulation of glutamine synthetase and g

Regulation of glutamine synthetase and glutaminase activities in cultured skeletal muscle cells

✍ Robert J. Smith; Sandra Larson; Susan E. Stred; Roberta P. Durschlag 📂 Article 📅 1984 🏛 John Wiley and Sons 🌐 English ⚖ 924 KB

## Abstract Glutamine is synthesized in skeletal muscle, released to the circulation, and transported to other tissues, where it may provide important substrate for gluconeogenesis, ammoniagenesis, and energy‐yielding pathways. With the ultimate goal of delineating the factors that control glutamin

Colorimetric Detection of Glutamine Synt

Colorimetric Detection of Glutamine Synthetase-Catalyzed Transferase Activity in Glucocorticoid-Treated Skeletal Muscle Cells

✍ Joseph C. Santoro; Georgianna Harris; Ayesha Sitlani 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 126 KB

Induction of the enzyme glutamine synthetase (GS) by corticosteroids correlates with muscle wasting and gluconeogenesis, characteristic side effects of chronic glucocorticoid treatment. This highlights the importance of developing robust high-throughput assays to measure drug-induced GS in whole cel

BDNF regulates GLAST and glutamine synth

BDNF regulates GLAST and glutamine synthetase in mouse retinal Müller cells

✍ MIN DAI; XIAO-BO XIA; SI-QI XIONG 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 459 KB

This study investigated whether brain-derived neurotrophic factor (BDNF) regulates the L-glutamate/L-aspartate transporter (GLAST) and glutamine synthetase (GS) in mouse retinal Mu ¨ller cells (RMCs) under normal and hypoxic conditions. Mouse RMCs were treated with recombinant human BDNF (50, 75, 10

Glutamine synthetase activity in WI-38 c

Glutamine synthetase activity in WI-38 cells

✍ Dace Viceps; V. J. Cristofalo 📂 Article 📅 1975 🏛 John Wiley and Sons 🌐 English ⚖ 325 KB

## Abstract In the presence of complete growth media (Eagle's MEM), human diploid WI‐38 cells have a low level of glutamine synthetase activity. The activity could be increased by depriving the cells of exogenous glutamine; addition of hydrocortisone to either glutamine‐deficient or complete medium

Regulation of glutamine synthetase in no

Regulation of glutamine synthetase in normal and injured neural tissues

✍ Lily Vardimon; Iris Ben Dror; Noa Avisar; Liora Shiftan; Yelena Kruchkova; Anat 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 209 KB 👁 1 views

Neuron–glial interactions involved in th

Neuron–glial interactions involved in the regulation of glutamine synthetase

✍ Karen M. Mearow; John F. Mill; Ernst Freese 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 876 KB

Cocultures of rat cortical astrocytes with cerebellar granule cell neurons, but not a variety of other cell types tested, resulted in an induction of glutamine synthetase (GS) mRNA over the basal levels expressed in pure astrocyte cultures. This induction involved both contact-and noncontact-mediate