BDNF regulates GLAST and glutamine synthetase in mouse retinal Müller cells

✍ Scribed by MIN DAI; XIAO-BO XIA; SI-QI XIONG

• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 459 KB
• Volume: 227
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.22762

✦ Synopsis

This study investigated whether brain-derived neurotrophic factor (BDNF) regulates the L-glutamate/L-aspartate transporter (GLAST) and glutamine synthetase (GS) in mouse retinal Mu ¨ller cells (RMCs) under normal and hypoxic conditions. Mouse RMCs were treated with recombinant human BDNF (50, 75, 100, 125, or 150 ng/ml) for 24 h or underwent hypoxia induced by CoCl 2 (125 mM; 6, 12, 24, 48, or 72 h). An additional group underwent combined treatment with BDNF (100 ng/ml; 24, 48, 72, or 96 h) and CoCl 2 (125 mM/ml; 72 h). GLAST and GS mRNA and protein expression, L-[3,4-3H]-glutamic acid uptake, and apoptosis were assessed. BDNF dose-dependently up-regulated GLAST and GS mRNA and protein and increased glutamate uptake. Similarly, in early-stage CoCl 2 -induced hypoxia, GLAST and GS were up-regulated and glutamate uptake increased, but these decreased over time. BDNF also up-regulated GLAST and GS and increased glutamate uptake when RMCs under CoCl 2 induced hypoxic condition. However, BDNF treatment 24 h before CoCl 2 had no effect on GLAST or GS expression. CoCl 2 alone or combined with BDNF did not induce apoptosis. Hypoxia rapidly increased GLAST and GS expressions. This effect was transient, perhaps due to compensatory mechanisms that reduce GLAST and GS by 72 h. BDNF can upregulate GLAST and GS and increase glutamate uptake during hypoxia, and these functions may underlie its neuroprotective effects.