Glutamate Receptors: Methods and Protocols

✍ Scribed by Corinna Burger, Margaret Jo Velardo

• Publisher: Springer New York;Humana Press
• Year: 2019
• Tongue: English
• Leaves: 257
• Series: Methods in Molecular Biology 1941
• Edition: 1st ed.
• Category: Library

✦ Synopsis

This volume aims to provide clear and detailed methods to probe glutamate receptor function. Chapters in this book feature methods to study synaptic ultrastructure, receptor dynamics/receptor interactions and trafficking, cellular plasticity, receptor gene regulation, epigenetics, and clinical applications. In addition, it includes overview chapters that provide critical reviews and historical perspectives on particular techniques and fields of study. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Glutamate Receptors: Methods and Protocols covers well-established glutamate receptor techniques, as well as new and cutting-edge techniques designed so that students, researchers, and clinicians can gain an understanding of the issues surrounding each experimental technique and its potential applications.

✦ Table of Contents

Front Matter ....Pages i-x
Front Matter ....Pages 1-1
Single-Molecule FRET Methods to Study Glutamate Receptors (Douglas B. Litwin, Ryan J. Durham, Vasanthi Jayaraman)....Pages 3-16
Ultrastructural Observation of Glutamatergic Synapses by Focused Ion Beam Scanning Electron Microscopy (FIB/SEM) (Ai Takahashi-Nakazato, Laxmi Kumar Parajuli, Hirohide Iwasaki, Shinji Tanaka, Shigeo Okabe)....Pages 17-27
Considerations for Imaging and Analyzing Neural Structures by STED Microscopy (Martin O. Lenz, Jan Tønnesen)....Pages 29-46
Cell-Based Enzyme-Linked Immunosorbent Assay (Cell-ELISA) Analysis of Native and Recombinant Glutamate Receptors (Elek Molnár)....Pages 47-54
Front Matter ....Pages 55-55
Preparation of Organotypic Slice Cultures for the Study of Glutamate Receptor Function (Andres Barria)....Pages 57-64
Glutamate Receptor Probing with Rapid Application and Solution Exchange (RASE) (Nathanael O’Neill, Sergiy Sylantyev)....Pages 65-78
Electrophysiological Investigation of Metabotropic Glutamate Receptor-Dependent Metaplasticity in the Hippocampus (Regina U. Hegemann, Wickliffe C. Abraham)....Pages 79-91
Induction of Metabotropic Glutamate Receptor-Mediated Long-Term Depression in the Hippocampal Schaffer Collateral Pathway of Aging Rats (Kirstan E. Gimse, Kenneth O’Riordan, Corinna Burger)....Pages 93-105
Whole-Cell Patch-Clamp Electrophysiology to Study Ionotropic Glutamatergic Receptors and Their Roles in Addiction (Jonna M. Leyrer-Jackson, M. Foster Olive, Cassandra D. Gipson)....Pages 107-135
Front Matter ....Pages 137-137
Gene Expression Analysis by Multiplex Single-Cell RT-PCR (Ludovic Tricoire, Bruno Cauli, Bertrand Lambolez)....Pages 139-154
Metabolomics Analysis of Glutamate Receptor Function (Nataliya E. Chorna, Anatoliy P. Chornyy)....Pages 155-165
Locus-Specific DNA Methylation Assays to Study Glutamate Receptor Regulation (Jordan A. Brown, J. David Sweatt, Garrett A. Kaas)....Pages 167-188
Preparation of Synaptoneurosomes for the Study of Glutamate Receptor Function (Cara J. Westmark, Pamela R. Westmark)....Pages 189-197
Front Matter ....Pages 199-199
Fractionation of Subcellular Compartments from Human Brain Tissue (Toni M. Mueller, Pitna Kim, James H. Meador-Woodruff)....Pages 201-223
Glutamate Receptor Antibodies in Autoimmune Central Nervous System Disease: Basic Mechanisms, Clinical Features, and Antibody Detection (William J. Scotton, Abid Karim, Saiju Jacob)....Pages 225-255
Back Matter ....Pages 257-260

✦ Subjects

Biomedicine; Neurosciences