Opioid Receptors: Methods and Protocols
β Santi M. Spampinato
π Library
π English
β¦ LIBER β¦
π
Opioid Receptors: Methods and Protocols
β Scribed by Santi M. Spampinato
- Publisher
- Springer US;Humana
- Year
- 2021
- Tongue
- English
- Leaves
- 268
- Series
- Methods in Molecular Biology 2201
- Edition
- 2nd ed.
- Category
- Library
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No coin nor oath required. For personal study only.
β¦ Synopsis
This second edition presents an up-to-date chapters describing the most relevant and novel techniques employed to study the opioid receptors. Chapters detail transcriptional and post-transcriptional analysis, cellular detection of opioid receptors, analysis of signaling events modulated by opioid receptors, model systems to studying opioid receptor-mediated functions, and behavioral effects mediated by opioid receptors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Opioid Receptors: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
β¦ Table of Contents
Front Matter ....Pages i-xv
Front Matter ....Pages 1-1
Overview of Genetic Analysis of Human Opioid Receptors (Santi M. Spampinato)....Pages 3-13
Renilla Luciferase Reporter Assay to Study 3β²-UTR-Driven Posttranscriptional Regulation of OPRM1 (Gabriele Vincelli, Andrea Bedini)....Pages 15-26
Fluorescence Colocalization Analysis of Cellular Distribution of MOR-1 (Vittoria Borgonetti, Nicoletta Galeotti)....Pages 27-34
Monitoring Opioid Receptor Interaction in Living Cells by Bioluminescence Resonance Energy Transfer (BRET) (Monica Baiula)....Pages 35-43
Bioluminescence Resonance Energy Transfer (BRET) to Detect the Interactions Between Kappa Opioid Receptor and Nonvisual Arrestins (Andrea Bedini)....Pages 45-58
Functionalization and Bioconjugation of Nanoruby for Long-Term, Ultrasensitive Imaging of Ξu-Opioid Receptors (Rashmi Pillai, Mark Connor, Varun K. A. Sreenivasan)....Pages 59-70
Immunohistochemical Analysis of Opioid Receptors in Peripheral Tissues (Yvonne Schmidt, Halina Machelska)....Pages 71-82
Real-Time Quantitative Reverse Transcription PCR for Detection of Opioid Receptors in Immune Cells (Melih ΓzgΓΌr Celik, Dominika Labuz, Halina Machelska)....Pages 83-95
Front Matter ....Pages 97-97
Quantitative Analysis of MOR-1 Internalization in Spinal Cord of Morphine-Tolerant Mice (Vittoria Borgonetti, Nicoletta Galeotti)....Pages 99-108
GTPΞ³S-Autoradiography for Studies of Opioid Receptor Functionality (Alfhild GrΓΆnbladh, Mathias Hallberg)....Pages 109-116
Evaluating Opioid-Mediated Adenylyl Cyclase Inhibition in Live Cells Using a BRET-Based Assay (Preeti Manandhar, Shivani Sachdev, Marina Santiago)....Pages 117-125
Patch Clamp Analysis of Opioid-Induced Kir3 Currents in Mouse Peripheral Sensory Neurons Following Nerve Injury (Viola Seitz, Philip StΓΆtzner, Dominika Labuz, Halina Machelska)....Pages 127-137
Opioid Modulation of Neuronal Iron and Potential Contributions to NeuroHIV (Bradley Nash, Elena Irollo, Renato Brandimarti, Olimpia Meucci)....Pages 139-162
An in Vitro Assay to Study the Role of Opioids in Modulating Immune Cell Adhesion (Monica Baiula)....Pages 163-169
Assessing Cell Viability Effects of Opioids in Primary Cortical Cells from Rat (Alfhild GrΓΆnbladh, Erik Nylander, Sofia Zelleroth, Mathias Hallberg)....Pages 171-180
The Quest for More Effective Analgesics with Reduced Abuse Liability and Fewer Adverse Effects: Promises, Pitfalls, and Future Perspectives of Biased Agonists at Opioid Receptors (Andrea Bedini)....Pages 181-192
Front Matter ....Pages 193-193
Mechanical Nociception in Mice and Rats: Measurement with Automated von Frey Equipment (Gabriele Campana, Roberto Rimondini)....Pages 195-198
Evaluation of Murine Macrophage Cytokine Production After In Vivo Morphine Treatment (Silvia Franchi, Mara Castelli, Sarah Moretti, Alberto Panerai, Paola Sacerdote)....Pages 199-207
Measurement of Macrophage Toll-Like Receptor 4 Expression After Morphine Treatment (Mara Castelli, Alberto Panerai, Paola Sacerdote, Silvia Franchi)....Pages 209-217
Front Matter ....Pages 219-219
Conditioned Place Preference (CPP) in Rats: From Conditioning to Reinstatement Test (Maria Scherma, Liana Fattore, Walter Fratta, Paola Fadda)....Pages 221-229
Analysis of Opioid-Seeking Behavior Through the Intravenous Self-Administration Reinstatement Model in Rats (Liana Fattore, Paola Fadda, Mary Tresa Zanda, Walter Fratta)....Pages 231-245
Induction of a High Alcohol Consumption in Rats and Mice: Role of Opioid Receptors in Rats and Mice (Roberto Rimondini, Gabriele Campana)....Pages 247-251
Early-Life Stress as a Probe to Study the Opioid System in Developing Rodents (Stefano Loizzo, Alberto Loizzo)....Pages 253-258
Evaluation of ΞΌ-Opioid System Functionality in Mouse Pups: Ultrasonic Vocalizations as an Index of Infant Attachment (Francesca R. DβAmato)....Pages 259-265
Back Matter ....Pages 267-270
β¦ Subjects
Life Sciences; Receptors; Neurobiology
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