NMDA Receptors: Methods and Protocols

✍ Scribed by Nail Burnashev, Pierre Szepetowski (eds.)

• Publisher: Humana Press
• Year: 2017
• Tongue: English
• Leaves: 316
• Series: Methods in Molecular Biology 1677
• Edition: 1
• Category: Library

✦ Synopsis

This volume explores the latest techniques used by researchers to help them better understand the NMDAR structure-function relations, principles, and rules that govern how NMDARs operate in brain processing. The volume includes a detailed introductory chapter describing the field and is divided into three parts. Topics covered in the volume include: quantification of NMDAR subunit genes expression by qRT-PCR; detection of NMDARs antibodies in encephalitis; recombinant channels in host cells using a fast agonist application system; GluNs detection and functions in microglial cells; and NMDARs as voltage sensors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, NMDA Receptors: Methods and Protocols is a valuable resource that discusses the diversity of the currently-used methods, the importance of NMDARs and their complexity, and the progress that has already been made. This book will be of interest to scientists, clinicians, and industry professionals working in this field.

✦ Table of Contents

Front Matter ....Pages i-xiii
NMDA Receptors in the Central Nervous System (Kasper B. Hansen, Feng Yi, Riley E. Perszyk, Frank S. Menniti, Stephen F. Traynelis)....Pages 1-80
Front Matter ....Pages 81-81
Quantification of NMDAR Subunit Genes Expression by qRT-PCR (Emilie Pallesi-Pocachard)....Pages 83-92
Genetic and Functional Analysis of GRIN2A in Tumor Samples (Todd D. Prickett, Jared J. Gartner, Yardena Samuels)....Pages 93-116
Detection of NMDARs Antibodies in Encephalitis (Matteo Gastaldi, Patrick Waters, Angela Vincent)....Pages 117-126
Front Matter ....Pages 127-127
Magnetofection™ of NMDA Receptor Subunits GluN1 and GluN2A Expression Vectors in Non-Neuronal Host Cells (Nadine Bruneau, Pierre Szepetowski)....Pages 129-135
Transfection in Primary Cultured Neuronal Cells (Katie F. M. Marwick, Giles E. Hardingham)....Pages 137-144
Selective Cell-Surface Expression of Triheteromeric NMDA Receptors (Feng Yi, Stephen F. Traynelis, Kasper B. Hansen)....Pages 145-162
Functional Analysis of Recombinant Channels in Host Cells Using a Fast Agonist Application System (Andrei Rozov, David Jappy)....Pages 163-169
GluN2B Subunit Labeling with Fluorescent Probes and High-Resolution Live Imaging (Cécile Perrio, Olivier Nicole, Alain Buisson)....Pages 171-183
Design of Light-Sensitive NMDARs by Genetically Encoded Photo-Cross-Linkers (Meilin Tian, Shixin Ye)....Pages 185-197
Front Matter ....Pages 199-199
Gene Targeted Mice with Conditional Knock-In (-Out) of NMDAR Mutations (Rolf Sprengel, Ahmed Eltokhi, Frank N. Single)....Pages 201-230
Electrophysiological Investigation of NMDA Current Properties in Brain Slices (Alexandre Pons-Bennaceur, Natalia Lozovaya)....Pages 231-239
Analysis of Functional NMDA Receptors in Astrocytes (Frank Kirchhoff)....Pages 241-251
GluNs Detection and Functions in Microglial Cells (Stéphane Peineau, Vincent Degos, Catherine Verney, Pierre Gressens)....Pages 253-263
NMDA Receptor Activity in Circulating Red Blood Cells: Methods of Detection (Asya Makhro, Lars Kaestner, Anna Bogdanova)....Pages 265-282
NMDA Receptors as Voltage Sensors (Roustem Khazipov)....Pages 283-290
Development of a Computational Approach/Model to Explore NMDA Receptors Functions (A. Florence Keller, Jean-Marie C. Bouteiller, Theodore W. Berger)....Pages 291-306
Back Matter ....Pages 307-314

✦ Subjects

Neurosciences