Glucose isomerase immobolized on porous glass

✍ Scribed by Y. Y. Lee; A. R. Fratzke; K. Wun; G. T. Tsao

• Publisher: John Wiley and Sons
• Year: 1976
• Tongue: English
• Weight: 952 KB
• Volume: 18
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260180309

✦ Synopsis

Abstract

Partially purified glucose isomerase from a Streptomyces species was immobilized on porous glass particles and studied for various characteristics concerning its use as an industrial catalyst. The activities were investigated in relation to the reaction parameters and the enzyme deactivation was studied systematically under various reaction conditions. The half‐life of the immobilized enzyme was found to exceed 200 days at 50°C. The rate equation of the reversible glucose ⇄ fructose reaction was derived and the kinetic constants were determined. The rate equation was found to be in good agreement with experimental data for both forward and reverse reactions. The degree of diffusional effects was experimentally measured and theoretically analyzed.