𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Genotypes and molecular epidemiology of human astroviruses in hospitalized children with acute gastroenteritis in Rio de Janeiro, Brazil

✍ Scribed by Matías Victoria; Filipe Aníbal Carvalho-Costa; Marcos Bryan Heinemann; José Paulo Gagliardi Leite; Marize Pereira Miagostovich

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
114 KB
Volume
79
Category
Article
ISSN
0146-6615

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Human astrovirus (HAstV) is an important pathogen that causes acute gastroenteritis mainly in children up to 5 years old worldwide. A total of 318 fecal samples were collected from January to December of 2004 from children with acute gastroenteritis hospitalized in Rio de Janeiro (RJ), Brazil. The samples were obtained from three public hospitals and were previously investigated for the presence of rotavirus (RV) and enteric adenovirus (AdV). The aim of this study was to determine the prevalence and molecular characterization of HAstV by reverse‐transcription polymerase chain reaction (RT‐PCR) and partial sequencing of open reading frame 2 (ORF2). HAstV infections were detected in 43 (14%) of the samples, of which 19 (6%) were mixed infections with HAstV and RV. HAstV were identified in all age groups, with 85% of the cases occurring in children younger than 2 years old. HAstV infections were more prevalent from March to May. Diarrhea, vomiting, and fever were the main clinical manifestations observed in these infections. Partial capsid sequencing of 35 HAstV strains characterized 25 (71%) as genotype 1 (HAstV‐1), 6 (17%) HAstV‐2, 3 (9%) HAstV‐8, and 1 (3%) HAstV‐4. Based on the genetic variability, three lineages were observed between RJ HAstV‐1 strains. This study demonstrated the importance of HAstV infections causing acute infantile gastroenteritis in hospitalized children in RJ, Brazil. Molecular epidemiological surveillance is helpful for designing prevention strategies of HAstV transmission. J. Med. Virol. 79:939–944, 2007. © 2007 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Adenoviruses in faeces of children with
Adenoviruses in faeces of children with acute gastroenteritis in Rio De Janeiro, Brazil
✍ J. P. G. Leite; H. G. Pereira; R. S. Azeredo; H. G. Schatzmayr 📂 Article 📅 1985 🏛 John Wiley and Sons 🌐 English ⚖ 415 KB

## Abstract Faeces from 746 children less than 5 years old with acute gastroenteritis were screened for the presence of adenovirus particles or antigens by immunoelectron microscopy (IEM) and enzyme immunoassay (EIA). Thirty‐five samples were positive by both IEM and EIA, two only by IEM, and two o

Prevalence of human astrovirus genotypes
Prevalence of human astrovirus genotypes associated with acute gastroenteritis among children in Belém, Brazil
✍ Yvone B. Gabbay; Alexandre C. Linhares; Elielma L. Cavalcante-Pepino; Liliany S. 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 245 KB

## Abstract Worldwide human astroviruses (HAstV) have increasingly been recognized as causative agents of viral gastroenteritis, mainly in infants and young children. The aim of this study was to assess the epidemiology and genotype diversity of HAstVs detected in children who participated in a tri

Detection and characterisation of human
Detection and characterisation of human astroviruses in children with acute gastroenteritis in Blantyre, Malawi
✍ Nigel A. Cunliffe; Winifred Dove; Jailosi S. Gondwe; Benson D.M. Thindwa; Julie 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 61 KB 👁 1 views

## Abstract In a 2‐year hospital‐based study of paediatric gastroenteritis in Blantyre, Malawi, astroviruses were detected by enzyme immunoassay in 15 (1.9%) of 786 inpatients and in 9 (2.3%) of 400 outpatients. Greater disease severity was noted in children coinfected with human immunodeficiency v

Molecular epidemiology of human adenovir
Molecular epidemiology of human adenovirus isolated from children hospitalized with acute respiratory infection in São Paulo, Brazil
✍ Patrícia O. Moura; Adriana F. Roberto; Noely Hein; Evandro Baldacci; Sandra E. V 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 202 KB

## Abstract In a study of acute respiratory disease, two collections of nasopharyngeal aspirates (NPA) were obtained from children hospitalized at the Pediatric Clinic of the University Hospital, São Paulo, in 1995 and 2000. Adenovirus was detected in 33 (8.2%) of 401 children followed. These virus

Astrovirus detection in sporadic cases o
Astrovirus detection in sporadic cases of diarrhea among hospitalized and non-hospitalized children in Rio De Janeiro, Brazil, from 1998 to 2004
✍ Caroline Cordeiro Soares; Maria Carolina Maciel de Albuquerque; Adriana Gonçalve 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 149 KB

## Abstract We analyzed 379 stool samples collected from January 1998 through December 2004, from hospitalized and non‐hospitalized children with diarrhea in Rio de Janeiro, Brazil. These samples had prior negative results for other enteric viruses and bacterial pathogens. The specimens were analyz

Detection and characterization of human
Detection and characterization of human caliciviruses in hospitalized children with acute gastroenteritis in Blantyre, Malawi
✍ Winifred Dove; Nigel A. Cunliffe; Jailosi S. Gondwe; Robin L. Broadhead; Malcolm 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 119 KB

The human caliciviruses (HuCVs), including Norovirus and Sapovirus, are recognized causes of acute gastroenteritis in children and adults. A 1-year study was undertaken in Blantyre, Malawi, to examine the prevalence, and genetic diversity, of human caliciviruses (HuCVs) amongst children under 5 year