Adenoviruses in faeces of children with acute gastroenteritis in Rio De Janeiro, Brazil

✍ Scribed by J. P. G. Leite; H. G. Pereira; R. S. Azeredo; H. G. Schatzmayr

• Publisher: John Wiley and Sons
• Year: 1985
• Tongue: English
• Weight: 415 KB
• Volume: 15
• Category: Article
• ISSN: 0146-6615
• DOI: 10.1002/jmv.1890150213

✦ Synopsis

Abstract

Faeces from 746 children less than 5 years old with acute gastroenteritis were screened for the presence of adenovirus particles or antigens by immunoelectron microscopy (IEM) and enzyme immunoassay (EIA). Thirty‐five samples were positive by both IEM and EIA, two only by IEM, and two only by EIA, giving a total of 39 (5.2%) samples with positive results. Of these, 25 could be propagated in HEp2 cells and were neutralized by one of the antisera to adenovirus types 1 to 18. The remaining 14 samples could be propagated only in the 293 permanent line of human cells transformed by adenovirus type 5 DNA [Graham et al, 1977] and were not neutralized by antisera to adenovirus types 1 to 31. An EIA carried out by the antibody‐capture technique, using antiserum specific for “enteric” adenoviruses [Johansson et al, 1979], gave positive results with all isolates that could be propagated only in 293 cells and with none of those capable of growing in HEp2 cells.