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Genome mining for the discovery of new nitrilases in filamentous fungi

✍ Scribed by Ondřej Kaplan; Karel Bezouška; Anna Malandra; Alicja B. Veselá; Alena Petříčková; Jürgen Felsberg; Anna Rinágelová; Vladimír Křen; Ludmila Martínková


Publisher
Springer Netherlands
Year
2010
Tongue
English
Weight
169 KB
Volume
33
Category
Article
ISSN
0141-5492

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Methods for electrophoretic karyotyping of filamentous fungi in the genus Trichoderma were developed. These techniques permitted the separation and visualization of intact chromosomes from viable protoplasts. Three strains were analyzed: Trichoderma harzianum strains T12 his-2 and T95 lys-1, and a p