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Genome mining approach for the discovery of novel cytochrome P450 biocatalysts

✍ Scribed by Toshiki Furuya; Kuniki Kino

Publisher
Springer
Year
2010
Tongue
English
Weight
272 KB
Volume
86
Category
Article
ISSN
1432-0614

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l a 16 Scheme 4. a) [BuLi. T H E 90 min, -78'C; BzCl, pyridine, 12h, 20-C; 5% Na/Hg. 5h. -20 C. 3 5 % : h) Bu,NE THF, 1 h, 0'C. 85%; c) tiBuLi. CICO,Et, THF, 1 h, ~ 30 C. 8 5 % ; d ) morpholine. THF. 2h, reflux; 60% CH,CO,H, 2h. 5 0 Β°C 75%; e) TBDPSCI. imidazole. DMF. ?d, -60 C, 9 6 % ; f) Ac,O, pyr