Genetics:Direct Comparison of Detection Systems Used for the Development of Single-Cell Genetic Tests in Preimplantation Genetic Diagnosis
โ Scribed by Deborah L. Blake; Nicola L. Dean; Casey Knight; Seang Lin Tan; Asangla Ao
- Book ID
- 110307894
- Publisher
- Springer US
- Year
- 2001
- Tongue
- English
- Weight
- 432 KB
- Volume
- 18
- Category
- Article
- ISSN
- 1573-7330
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Preimplantation genetic diagnosis (PGD) allows the selection of unaffected IVF embryos for transfer in couples that are at risk for transmitting genetic diseases. For monogenic diseases, polymerase chain reaction (PCR)-based diagnosis is usually performed on single blastomeres. In Greece, up to 10 p
The combination of in vitro fertilization (IVF) with PCR technologies enables diagnosis of single gene defects for preimplantation genetic diagnosis. This has been accomplished by two-step nested PCR, or PEP-PCR followed by nested PCR processes. To improve the detection of single cell genetic defect