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Single cell co-amplification of polymorphic markers for the indirect preimplantation genetic diagnosis of hemophilia

✍ Scribed by Nadine Gigarel; Nelly Frydman; Philippe Burlet; Violaine Kerbrat; Julie Steffann; René Frydman; Arnold Munnich; PierreF. Ray


Book ID
106133933
Publisher
Springer
Year
2004
Tongue
English
Weight
299 KB
Volume
114
Category
Article
ISSN
0340-6717

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The combination of in vitro fertilization (IVF) with PCR technologies enables diagnosis of single gene defects for preimplantation genetic diagnosis. This has been accomplished by two-step nested PCR, or PEP-PCR followed by nested PCR processes. To improve the detection of single cell genetic defect