The combination of in vitro fertilization (IVF) with PCR technologies enables diagnosis of single gene defects for preimplantation genetic diagnosis. This has been accomplished by two-step nested PCR, or PEP-PCR followed by nested PCR processes. To improve the detection of single cell genetic defect
Reduced allele dropout in single-cell analysis for preimplantation genetic diagnosis of cystic fibrosis
โ Scribed by Pierre F. Ray; Robert M. L. Winston; Alan H. Handyside
- Book ID
- 105451320
- Publisher
- Springer US
- Year
- 1996
- Tongue
- English
- Weight
- 311 KB
- Volume
- 13
- Category
- Article
- ISSN
- 1573-7330
No coin nor oath required. For personal study only.
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Following the birth of a baby girl confirmed to be homozygous normal for the delta F508 deletion causing cystic fibrosis (CF), many single-gene defects have been diagnosed by polymerase chain reaction (PCR) for preimplantation genetic diagnosis (PGD). A few misdiagnoses have been reported but no lar
Preimplantation genetic diagnosis (PGD) allows the selection of unaffected IVF embryos for transfer in couples that are at risk for transmitting genetic diseases. For monogenic diseases, polymerase chain reaction (PCR)-based diagnosis is usually performed on single blastomeres. In Greece, up to 10 p