Genetically engineered avidins and streptavidins
✍ Scribed by O. H. Laitinen; V. P. Hytönen; H. R. Nordlund; M. S. Kulomaa
- Publisher
- Springer
- Year
- 2006
- Tongue
- English
- Weight
- 667 KB
- Volume
- 63
- Category
- Article
- ISSN
- 1420-682X
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📜 SIMILAR VOLUMES
Underestimation of avidin and streptavidin by the Bradford method can be alleviated by carrying out the reaction at 100 degrees C for 10-15 min, and the protein values thus obtained match very well with those of biotin-binding assays. "Thermal modification" is applicable to "standard" and "micro" ve
## Abstract Avidin and streptavidin were studied by phase‐sensitive AC and cyclic voltammetry as well as by constant current chronopotentiometry at mercury (in alkaline media) and carbon electrodes (in acid medium). In contrast to the generally accepted belief that these proteins are electroinactiv