The quenching of the tryptophan fluorescence of avidin by biotin forms the basis for a rapid, sensitive assay for biotin and avidin in solutions free of fluorescent contaminants. Avidin solutions are assayed by titration with a standard biotin solution until no further decrease in fluorescence at 35
A sensitive enzyme assay for biotin, avidin, and streptavidin
β Scribed by Edward A. Bayer; Haya Ben-Hur; Meir Wilchek
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 299 KB
- Volume
- 154
- Category
- Article
- ISSN
- 0003-2697
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A unique homogeneous enzyme immunoassay used for the determination of haptens and macromolecular antigens has been developed. It consists of avidin-antigen conjugate, anti-ligand antibody, and biotin enzyme (propionyl-CoA carboxylase). The immunological reaction of the conjugate with antibodies spec
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