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Determination of avidin and streptavidin by a modified Bradford method

✍ Scribed by Harmesh K. Sharma; Claude Tihon

Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
350 KB
Volume
170
Category
Article
ISSN
0003-2697

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✦ Synopsis

Underestimation of avidin and streptavidin by the Bradford method can be alleviated by carrying out the reaction at 100 degrees C for 10-15 min, and the protein values thus obtained match very well with those of biotin-binding assays. "Thermal modification" is applicable to "standard" and "micro" versions of the method and has no effect on the spectral and stability characteristics of the protein-dye complexes.

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