Genetic variability of alkaline phosphatase expression in inbred mouse tissues

✍ Scribed by David J. Goldstein; Richard Levy; Pao-Lo Yu; Harry Harris

• Publisher: Springer
• Year: 1985
• Tongue: English
• Weight: 549 KB
• Volume: 23
• Category: Article
• ISSN: 0006-2928
• DOI: 10.1007/bf00499120

✦ Synopsis

Quantitative alkaline phosphatase (ALP; EC 3.1.3.1)

expression varies among various tissues and among inbred mouse strains.

There is about a 20-fold difference in ALP activity in lungs from CBA/J and C57L/J inbred strains and this difference is inherited additively with a heritability of 0.84. Studies of thermostability at 56 and 65 °C and sensitivity toward inhibitors (L-phenylalanine, L-homoarginine, L-phenylalanylglycylglycine, and levamisole) do not demonstrate differences in the ALP from lungs or liver of the CBA/J and C57L/J strains. The ALP activity in intestine expressed by the intestinal locus varies over lO0-fold between A/J and DBA/1J strains. Further studies of the mechanisms resulting in this difference in ALP activity should help elucidate the mechanisms for aberrant expression of ALP in malignancy and for manipulation o flow ALP activity in hypophosphatasia.