A cloned 850 bp cDNA fragment corresponding to the 3'-coding part of human ALDHI-mRNA was used as a probe for the chromosomal assignment of the ALDHI gene. Southern blot analysis of human-rodent somatic cell hybrids indicates that the human ALDHI gene resides on chromosome 12.
Genetic mapping of the structural gene for antithrombin III to human chromosome 1
โ Scribed by F. T. Kao; H. G. Morse; M. L. Law; A. Lidsky; T. Chandra; S. L. C. Woo
- Publisher
- Springer
- Year
- 1984
- Tongue
- English
- Weight
- 508 KB
- Volume
- 67
- Category
- Article
- ISSN
- 0340-6717
No coin nor oath required. For personal study only.
โฆ Synopsis
Using a purified cDNA probe of human antithrombin III (AT3) gene and a series of human/Chinese hamster cell hybrids, we established the chromosomal location of the structural gene for AT3 in human chromosome 1 by Southern blot analysis.
๐ SIMILAR VOLUMES
A 353-bp region encoding for the NH2 terminus of the noncollagenic part of the alpha 1(V) chain was amplified by the polymerase chain reaction (PCR), subcloned and sequenced. The subcloned PCR product (pGC1) presented the same nucleotide sequence as the original fragment from the published sequence
## Method of mapping: 113 animals from the backcross (DP-BB โข DR-BB)F 1 โข DP-BB were analyzed. Molecular reagent: Primer sequences used for PCR were a sense primer, 5'-CTCCCAGCTGTGGATGGGTTC-3' (nucleotide position 3134 to 3154 of mouse Hoxall homeobox gene), and an anti-sense primer, 5'-CCAGAACAGC