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Genetic mapping of the structural gene for antithrombin III to human chromosome 1

โœ Scribed by F. T. Kao; H. G. Morse; M. L. Law; A. Lidsky; T. Chandra; S. L. C. Woo


Publisher
Springer
Year
1984
Tongue
English
Weight
508 KB
Volume
67
Category
Article
ISSN
0340-6717

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โœฆ Synopsis


Using a purified cDNA probe of human antithrombin III (AT3) gene and a series of human/Chinese hamster cell hybrids, we established the chromosomal location of the structural gene for AT3 in human chromosome 1 by Southern blot analysis.


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## Method of mapping: 113 animals from the backcross (DP-BB โ€ข DR-BB)F 1 โ€ข DP-BB were analyzed. Molecular reagent: Primer sequences used for PCR were a sense primer, 5'-CTCCCAGCTGTGGATGGGTTC-3' (nucleotide position 3134 to 3154 of mouse Hoxall homeobox gene), and an anti-sense primer, 5'-CCAGAACAGC