Genetic manipulation of microspores and microspore-derived embryos
โ Scribed by Bin Huang
- Book ID
- 112903163
- Publisher
- Society for In Vitro Biology
- Year
- 1992
- Tongue
- English
- Weight
- 663 KB
- Volume
- 28
- Category
- Article
- ISSN
- 1475-2689
No coin nor oath required. For personal study only.
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An in vitro culture system has been developed for barley (Hordeum vulgare), which yields high frequencies of high quality microspore-derived embryos without an intervening callus phase. The embryos are very similar to zygotic embryos with regard to their morphology and germination capacity. These em
Microspore-derived embryos from Brassica napus L. were dried to less than 15% moisture and stored dry for a minimum of 7 days. Successful plant regeneration was observed when embryos at the cotyledonary stage of development were treated with 50 uM ABA for 7 days prior to desiccation. Solid agar or l