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Efficient isolation of microspores and the production of microspore-derived embryos fromBrassica napus

✍ Scribed by Eric B. Swanson; Marc P. Coumans; Sau Ching Wu; Tina L. Barsby; W. D. Beversdorf

Book ID
104699269
Publisher
Springer
Year
1987
Tongue
English
Weight
488 KB
Volume
6
Category
Article
ISSN
0721-7714

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Induction of freezing tolerance in microspore-derived embryos of winterBrassica napus
✍ W. Orr; A. M. Johnson-Flanagan; W. A. Keller; J. Singh πŸ“‚ Article πŸ“… 1990 πŸ› Springer 🌐 English βš– 585 KB

Freezing tolerance was induced in microspore derived embryos of winter Brassica napus cv. Jet neuf by the addition of ABA or mefluidide to the culture media during embryogenesis. Survival after freezing was estimated by culture of frozen-thawed embryos to plantlets. A higher freezing tolerance (50%