Microspore-derived embryo induction from cultured anthers of wheat
β Scribed by H.M. Ingram; J.B. Power; K.C. Lowe; M.R. Davey
- Book ID
- 110235012
- Publisher
- Springer
- Year
- 2000
- Tongue
- English
- Weight
- 39 KB
- Volume
- 60
- Category
- Article
- ISSN
- 0167-6857
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A period of four days preincubation at 25 Β°C on a medium containing mannitol was found to be superior to those pretreatments requiring incubation at 4 Β°C. In addition, the yield of green plants was improved by orienting anthers 'flat' on the medium during mannitol preincubation, and reducing the num
In a previous study on the RFLP analysis of the maize anther culture response (Wan et al. 1992), some of the anther-derived callus Unes from hybrids H99 x Pa91 (HP) and H99 x FR16 (HF) showed the same RFLP patterns with 58 (for HP Unes) or 35 (for HF lines) RFLP markers used. Since the callus Unes w