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Genetic and biochemical characterization of phosphofructokinase fromDrosophila melanogaster

โœ Scribed by Lori R. Munneke; Glen E. Collier

Publisher
Springer
Year
1985
Tongue
English
Weight
549 KB
Volume
23
Category
Article
ISSN
0006-2928

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โœฆ Synopsis

Phosphofructokinase (PFK;EC 2.7.1.11) activity in Drosophila melanogaster is controlled by a single dosage-sensitive region of the genome between 45F and 47E of chromosome IIR. Only a single form of PFK was detected electrophoretically in both adults and larvae. Nearly 90% of the PFK activity in adults is localized to the thorax. Purification of the enzyme was hampered by the extreme lability of Drosophila PFK; however, a 36-fold partial purification was achieved.

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