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Purification and genetic control of NAD-dependent glutamate dehydrogenase fromDrosophila melanogaster

✍ Scribed by C. Caggese; V. Pinto; A. Ferrandino

Publisher
Springer
Year
1982
Tongue
English
Weight
499 KB
Volume
20
Category
Article
ISSN
0006-2928

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✦ Synopsis

Glutamate dehydrogenase has been purified to near-homogeneity from mature larvae of Drosophila melanogaster. The enzyme has a molecular weight of 347,000 measured by sucrose gradient sedimentation and 343,000 measured by variable-porosity acrylamide gel electrophoresis. Electrophoresis under denaturing conditions showed that the enzyme consists of six subunits of molecular weight 57,000. The structural gene for GDH has been mapped at 81.7 +_ 0.8 on the third chromosome by means of an electrophoretic variant.

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