Isoelectrofocusing of abdominal extracts of Drosophila melanogaster revealed the existence of two forms of sucrase (E.C. 3.2.1.26). One form exhibited an isoelectric point of 4.63 +/- 0.02 while the other form exhibited an isoelectric point of 4.83 +/- 0.02. The localization of the structural gene f
Genetic and biochemical aspects of trehalase fromDrosophila melanogaster
โ Scribed by Melvin J. Oliver; R. E. Huber; John H. Williamson
- Publisher
- Springer
- Year
- 1978
- Tongue
- English
- Weight
- 686 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0006-2928
No coin nor oath required. For personal study only.
โฆ Synopsis
Only one molecular form of trehalase (E.C. 3.2.1.28) was detectable in adult Drosophila melanogaster by polyacrylamide gel electrophoresis and isoelectric focusing. An examination of duplication- and deletion-bearing aneuploids exhibiting dosage sensitivity indicated that the enzyme is encoded by a gene, Treh+, located between 55B and 55E of the second chromosome. The tissue-specific soluble and particulate forms of trehalase appear to be manifestations of a single protein encoded by a single gene.
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