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Generation of a floxed allele of the mouse BMP type II receptor gene

✍ Scribed by Hideyuki Beppu; Hong Lei; Kenneth D. Bloch; En Li

Publisher: John Wiley and Sons
Year: 2005
Tongue: English
Weight: 292 KB
Volume: 41
Category: Article
ISSN: 1526-954X
DOI: 10.1002/gene.20099

No coin nor oath required. For personal study only.

✦ Synopsis

Bone morphogenetic proteins (BMPs) regulate a wide range of cellular functions that contribute to embryonic development from mesoderm formation to organogenesis. BMP type II receptor (BMPR-II) transduces BMP signals by forming heteromeric complexes with and phosphorylating BMP type I receptors. Heterozygous germline mutations of BMPR-II gene have been identified in patients with familial and sporadic primary pulmonary hypertension, indicating that BMPR-II may contribute to the maintenance of normal pulmonary vascular structure and function. Since embryos homozygous for a null BMPR-II allele died during gastrulation, precluding further studies of BMPR-II function in organ formation and in adult tissues, we generated mice carrying a conditional mutant BMPR-II allele in which exons 4 and 5 were flanked by loxP sequences. We anticipate that studies of mice carrying a floxed BMPR-II allele and a Cre transgene (under the control of a tissue-specific promoter) will enable characterization of the role of BMPR-II in specific cell types during development and in the pathogenesis of cardiovascular diseases. genesis 41: 133-137, 2005.

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