Generation of a conditional allele of the mouse prostaglandin EP4 receptor

✍ Scribed by André Schneider; YouFei Guan; Yahua Zhang; Mark A. Magnuson; Cathy Pettepher; Charles D. Loftin; Robert Langenbach; Richard M. Breyer; Matthew D. Breyer

• Publisher: John Wiley and Sons
• Year: 2004
• Tongue: English
• Weight: 379 KB
• Volume: 40
• Category: Article
• ISSN: 1526-954X
• DOI: 10.1002/gene.20048

✦ Synopsis

Abstract

Genetic disruption of the mouse EP~4~ receptor results in perinatal lethality associated with persistent patent ductus areteriosus (PDA). To circumvent this, an EP~4~ allele amenable to conditional deletion using the Cre/loxP system was generated. The targeting construct was comprised of a floxed exon2 in tandem with the neomycin‐resistance gene in intron 2, flanked by third 3′ LoxP site. Mice homozygous for the targeted allele (EP~4~^lox+neo/lox+neo^), or following its Cre‐mediated deletion (EP~4~^del/del^), also die within hours of birth with PDA. In contrast, mice homozygous for a partially recombined allele, retaining exon2 but lacking neo (EP~4~^flox/flox^), are viable and show no overt phenotype. Postnatal deletion of the floxed EP~4~ gene is efficiently achieved in the liver and kidney in a transgenic mouse expressing the inducible Mx1__Cre__ recombinase. The EP mouse should provide a useful reagent with which to examine the physiologic roles of the EP~4~ receptor. genesis 40:7–14, 2004. © 2004 Wiley‐Liss, Inc.