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Generation of a floxed allele of Smad5 for cre-mediated conditional knockout in the mouse

✍ Scribed by Lieve Umans; Liesbeth Vermeire; Annick Francis; Hua Chang; Danny Huylebroeck; An Zwijsen

Publisher: John Wiley and Sons
Year: 2003
Tongue: English
Weight: 348 KB
Volume: 37
Category: Article
ISSN: 1526-954X
DOI: 10.1002/gene.10219

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Summary: Smad5 is a member of the Smad family of intracellular mediators of BMP signals and in endothelial cells of TGF‐β signals. We and others previously showed that loss of Smad5 in the mouse results in embryonic lethality (between E9.5–E11.5) due to multiple embryonic and extraembryonic defects. To circumvent the early embryonic lethality and to allow tissue‐ and time‐specific Smad5 inactivation, we created a conditional Smad5 allele in the mouse. Floxed Smad5 (Smad5^flE2,Neo/flE2,Neo^) mice were generated in which both exon2 and the Neo‐cassette were flanked by loxP sites. Here we demonstrate that embryos with ubiquitous Cre‐mediated deletion of Smad5 (Smad5^flΔE2/flΔE2^) phenocopy the conventional Smad5 knockout mice. Smad5^flE2/flE2^ mice are now available and will be a valuable tool to analyze the role of Smad5 beyond its crucial early embryonic function throughout development and postnatal life. genesis 37:5–11, 2003. © 2003 Wiley‐Liss, Inc.

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