Random mntagenesis of the gene for bacte
โ
Rechinsky, Vladimir O. ;Kostyuk, Dmitry A. ;Lyakhov, Dmitry L. ;Chernov, Boris K
๐
Article
๐
1993
๐
Springer
๐
English
โ 421 KB
Random mutagenesis of the gene for bacteriophage T7 RNA polymerase was used to identify functionally essential amino acid residues of the enzyme. A two-plasmid system was developed that permits the straightforward isolation of T7 RNA polymerase mutants that had lost almost all catalytic activity. It