Random mutagenesis of the gene for bacteriophage T7 RNA polymerase was used to identify functionally essential amino acid residues of the enzyme. A two-plasmid system was developed that permits the straightforward isolation of T7 RNA polymerase mutants that had lost almost all catalytic activity. It
Single crystals of bacteriophage T7 RNA polymerase
β Scribed by Rui Sousa; John P. Rose; Yong Je Chung; Eileen M. Lafer; Bi-Cheng Wang
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 509 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0887-3585
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## Abstract Dimethylsulfoxide (DMSO)Abbreviations used: Dimethylsulfoxide DMSO, polyvinyl sulfate PVS, nucleoside triphosphate NTP up to 25% (v/v) does not cause irreversible alterations of T3 DNA at 42.5Β°C as assayed by transcription with T3βspecific RNA polymerase. The optimal temperature for th
## Abstract Bacteriophage T3βinduced RNA polymerase is rapidly inactivated at 42Β°C. Addition of T3 DNA delays this process for 30 s and reduces the rate with which the enzyme activity is lsot indicating that a labile binary complex between T3 DNA and polymerase must have been formed. The ternary co