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Genetic analysis of two bacterial RNA polymerase mutants that inhibit the growth of bacteriophage T7

โœ Scribed by Buchstein, Sara R. ;Hinkle, David C.


Publisher
Springer
Year
1982
Tongue
English
Weight
824 KB
Volume
188
Category
Article
ISSN
0026-8925

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We describe a bacterial RNA polymerase mutation, rif 501, which confers rifampicin resistance and thermosensitivity to E. coli K 12. The purified RNA polymerase enzyme from rif 501 bacteria shows increased heatsensitivity in vitro at 51 degrees C. However, in vivo, at 42 degrees C the non-permissive