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Stimulation of RNA chain initiation by the N gene protein of bacteriophage λ

✍ Scribed by Wood, Karen C. ;Konrad, Michael W.


Book ID
104728890
Publisher
Springer
Year
1974
Tongue
English
Weight
528 KB
Volume
134
Category
Article
ISSN
0026-8925

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Random mutagenesis of the gene for bacteriophage T7 RNA polymerase was used to identify functionally essential amino acid residues of the enzyme. A two-plasmid system was developed that permits the straightforward isolation of T7 RNA polymerase mutants that had lost almost all catalytic activity. It