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Gene expression profiling using advanced mass spectrometric approaches

✍ Scribed by Ljiljana Paša-Tolić; Mary S. Lipton; Christophe D. Masselon; Gordon A. Anderson; Yufeng Shen; Nikola Tolić; Richard D. Smith

Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
761 KB
Volume
37
Category
Article
ISSN
1076-5174

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✦ Synopsis

Abstract

In the era of systems biology, computational and high‐throughput experimental biological approaches are increasingly being combined to provide global snapshots of entire genomes and proteomes under tissue‐ and disease‐specific conditions. The aim is to identify proteins changing in concentration and/or post‐translational state and/or location, and develop a better molecular level understanding of the operation of biological systems. Here we describe an approach for comparative proteomics that builds upon the combination of high‐performance nano‐scale separations with the high‐mass measurement accuracy, mass‐resolving power and sensitivity of Fourier transform ion cyclotron resonance mass spectrometry to provide broad dynamic range, comprehensive and quantitative proteome measurements. Copyright © 2002 John Wiley & Sons, Ltd.

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