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Gene cloning and characterization of an aldehyde dehydrogenase from long-chain alkane-degradingGeobacillusthermoleovoransB23

✍ Scribed by Tomohisa Kato; Asuka Miyanaga; Shigenori Kanaya; Masaaki Morikawa


Publisher
Springer
Year
2009
Tongue
English
Weight
631 KB
Volume
14
Category
Article
ISSN
1431-0651

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Long-chain alcohol dehydrogenase and longchain aldehyde dehydrogenase were induced in the cells of Candida tropicalis grown on n-alkanes. Subcellular localization of these dehydrogenases, together with that of acyl-CoA synthetase and glycerol-3-phosphate acyltransferase, was studied in terms of the

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The ACO3 gene, which encodes one of the acyl-CoA oxidase isoenzymes, was isolated from the alkane-utilizing yeast Yarrowia lipolytica as a 10 kb genomic fragment. It was sequenced and found to encode a 701-amino acid protein very similar to other ACOs, 67.5% identical to Y. lipolytica Aco1p and abou