Subcellular localization of long-chain alcohol dehydrogenase and aldehyde dehydrogenase inn-alkane-grownCandida tropicalis
✍ Scribed by Takao Yamada; Hiroyuki Nawa; Susumu Kawamoto; Atsuo Tanaka; Saburo Fukui
- Publisher
- Springer
- Year
- 1980
- Tongue
- English
- Weight
- 534 KB
- Volume
- 128
- Category
- Article
- ISSN
- 0302-8933
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✦ Synopsis
Long-chain alcohol dehydrogenase and longchain aldehyde dehydrogenase were induced in the cells of Candida tropicalis grown on n-alkanes. Subcellular localization of these dehydrogenases, together with that of acyl-CoA synthetase and glycerol-3-phosphate acyltransferase, was studied in terms of the metabolism of fatty acids derived from n-alkane substrates. Both longchain alcohol and aldehyde dehydrogenases distributed in the fractions of microsomes, mitochondria and peroxisomes obtained from the alkane-grown cells of C. tropicalis. Acyl-CoA synthetase was also located in these three fractions. Glycerol-3-phosphate acyltransferase was found in microsomes and mitochondria, in contrast to fatty acid /%oxidation system localized exclusively in peroxisomes. Similar results of the enzyme localization were also obtained with C. lipolytica grown on n-alkanes. These results suggest strongly that microsomal and mitochondrial dehydrogenases provide long-chain fatty acids to be utilized for lipid synthesis, whereas those in peroxisomes supply fatty acids to be degraded via/3-oxidation to yield energy and cell constituents.