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Gene amplification in multidrug-resistant cells: Molecular and karyotypic events

โœ Scribed by Andrey Gudkov; Boris Kopnin

Publisher
John Wiley and Sons
Year
1985
Tongue
English
Weight
659 KB
Volume
3
Category
Article
ISSN
0265-9247

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โœฆ Synopsis

Multidrug resistance (MDR) is developed in a population of somatic mammalian cells in vivo or in vitro when they are selected for resistance to each of a large group of drugs (colchicine, adriamycin, actinomycin D , etc.). It isproduced by the amplification of some unknown gene@) whose praduct(s) evidently change@) rhe plasma membrane permeability of a cell to a selective agent and to other unrelated compounds. A large spec$c genomic region (150-250 kbp) undergoes amplification in MDR cells selected for the resistance to diflerent drugs. Amplijied DNA is locatedin homogeneously staining regions of chromosomes, double minute chromosomes and small chromatin bodies

episome-like structures that replicate out of the S-phase of a cell cycle. The selection of MDR cell variants is accompanied by regular karyotypic evolution specijic for this type of drug resistance.

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