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Gel permeation chromatography applied to the study of the nitrohumic acids from coal

✍ Scribed by Rafael Moliner; J. Osácar; Jose Ma Gavilán

Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
449 KB
Volume
61
Category
Article
ISSN
0016-2361

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✦ Synopsis

Gel permeation chromatography on Sephadex (G-types) was applied to nitrohumic acids (NHA) from Iignites. Deionized water and TRIS (Tris-hydroxymethylaminomethane) buffer were tested as eluents. Deionized water provided the same number of fractions and better resolution than TRIS. In both cases, interactions between the gel matrix and the NHA occurred. Five NHA fractions were obtained and characterized by elemental and spectral analysis, which showed significant differences between them. Molecular weights of the fractions, calculated from Cameron's calibration for soil humic acids, are < 9000 or > 100 000 with no significant fractions having molecular weights in the range 9000-I 00 000.

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