✦ LIBER ✦

Gas-phase hydrolysis of proteins and peptides

✍ Scribed by Noel M. Meltzer; Guillermo I. Tous; Stephen Gruber; Stanley Stein

Publisher: Elsevier Science
Year: 1987
Tongue: English
Weight: 888 KB
Volume: 160
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(87)90060-1

No coin nor oath required. For personal study only.

✦ Synopsis

A simplified gas-phase hydrolysis procedure for proteins and peptides is described. The apparatus consists of a glass vacuum desiccator, a ceramic plate, and a Teflon ring. The method was shown to give reproducible compositions for hydrolysis of human serum albumin and microanalysis of a-melanocyte stimulating hormone including the quantitation of as little as one residue of tryptophan. It minimizes sample handling and allows for the simultaneous hydrolysis of a large number of samples.

📜 SIMILAR VOLUMES

Ion-molecule reactions as probes of gas-

Ion-molecule reactions as probes of gas-phase structures of peptides and proteins

✍ M. Kirk Green; Carlito B. Lebrilla 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 194 KB 👁 2 views

A review with over 100 references describes the recent applicatures. Unlike collision-induced dissociation or collisiontions of ion-molecule reactions to the study of gas-phase protonally activated dissociation, which probe the primary strucated peptides and proteins. The topic is focused specifical

Microsequence analysis of peptides and p

Microsequence analysis of peptides and proteins: IX. Manual gas-phase microsequencing of multiple samples

✍ Mitsuru Haniu; John E. Shively 📂 Article 📅 1988 🏛 Elsevier Science 🌐 English ⚖ 778 KB

A novel apparatus for performing manual gas-phase Edman chemistry on protein and peptide samples is described. Edman chemistry is performed in 6 to 10 Teflon continuous flow reactors (CFR), previously described by J. E. Shively et al. (1987) Anal. Biochem. 163, 5 17-529). The CFRs are packed with IO

Gas-phase fragmentation of protonated mo

Gas-phase fragmentation of protonated mono-N-methylated peptides. Analogy with solution-phase acid-catalyzed hydrolysis

✍ Vaisar, Tomáš; Urban, Jan 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 436 KB 👁 1 views

Fragmentation of protonated peptides with a single N-methylated residue was studied by low-energy collisioninduced dissociation (CID) and the e †ects of the N-alkylation on the fragmentation were evaluated. Peptides with an N-terminal N-alkylated amino acid behave similarly to regular peptides excep

Quantification of Glutamine in Proteins

Quantification of Glutamine in Proteins and Peptides Using Enzymatic Hydrolysis and Reverse-Phase High-Performance Liquid Chromatography

✍ Marlene Tsao; Don E. Otter 📂 Article 📅 1999 🏛 Elsevier Science 🌐 English ⚖ 63 KB

ChemInform Abstract: Ion-Molecule Reacti

ChemInform Abstract: Ion-Molecule Reactions as Probes of Gas-Phase Structures of Peptides and Proteins

✍ M. K. GREEN; C. B. LEBRILLA 📂 Article 📅 2010 🏛 John Wiley and Sons ⚖ 24 KB 👁 2 views

Hydrolysis and Formation of Peptides

✍ Hans-Dieter Jakubke 📂 Article 📅 2003 🏛 John Wiley and Sons ⚖ 49 KB

## Abstract For Abstract see ChemInform Abstract in Full Text.