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Gas-liquid chromatography of amino acids: Columns and methodology as a basis for routine amino acid analysis using glass capillary gas chromatography

✍ Scribed by Moodie, I. M. ;Burger, J.

Publisher: John Wiley and Sons
Year: 1981
Tongue: English
Weight: 423 KB
Volume: 4
Category: Article
ISSN: 0935-6304
DOI: 10.1002/jhrc.1240040505

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✦ Synopsis

Abstract

A carefully Standardized technique is described for the preparation of glass capillary columns which can be used successfully for routine quantitative amino acid analysis. Comparison is made between two different modes of sample injection. Preliminary quantitative results from “split” injection and “on‐column” injection techniques are evaluated statistically and it is concluded that the “on‐column” system is a prerequisite for quantitative amino acid analysis by glass capillary gas chromatography. An analysis of fish muscle protein hydrolyzate illustrates an application of this technique and results are compared with those from a packed column analysis.

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