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Gas chromatography of plasma amino acids using a wide bore column: Comparison with packed and capillary columns

✍ Scribed by Labadarios, D. ;Moodie, I. M. ;Burger, J. A. ;Shephard, G. S.

Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
351 KB
Volume
11
Category
Article
ISSN
0935-6304

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✦ Synopsis

A procedure is described in which a wide bore glass capillary column is used as an alternative to the more traditional packed column in the analysis of amino acid levels in plasma. The coefficientsof variationforallaminoacids (with theexceptionof aspartic acid) were better than 1 1 O h with recoveries ranging from 81% to 122OI0. The data are compared with the corresponding results obtained using a packed column and show significant differences (p < 0.005) between values for glycine, serine, isoleucine, proline, methionine, aspartic acid, phenylalanine, and lysine. A similar comparison between resultsfrom the wide bore and the fused-silica open tubular (FSOT) column shows better agreement. Adjustment of chromatographic conditions for the wide bore analysis yields results in good agreement with those from FSOT analysis but which still differ significantly from the packed column data.

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