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Determination of amino acids by gas chromatography using a wide bore capillary column

✍ Scribed by Moodie, I. M. ;Burger, J. A. ;Labadarios, D.

Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
429 KB
Volume
10
Category
Article
ISSN
0935-6304

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✦ Synopsis

A procedure is described in whicha wide borecapillarycolumn is used as an alternative to the more traditional packed column for the quantitative analysis of amino acids as their N-heptafluorobutyryl isobutyl ester (HBB) derivatives. Thecolumn, installed in a gas chromatograph previously configured for use with a packed column, is shown to give good reproducibility by repeated determination of amino acid response factors (RSD values for all amino acids are below 3%). A number of problems, encountered during the use of this column, are discussed and suitable techniques to overcome them are reported.

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