Gas-inducible transgene expression in mammalian cells and mice
β Scribed by Weber, Wilfried; Rimann, Markus; Spielmann, Manuela; Keller, Bettina; Baba, Marie Daoud-El; Aubel, Dominique; Weber, Cornelia C; Fussenegger, Martin
- Book ID
- 109908303
- Publisher
- Nature Publishing Group
- Year
- 2004
- Tongue
- English
- Weight
- 194 KB
- Volume
- 22
- Category
- Article
- ISSN
- 1087-0156
- DOI
- 10.1038/nbt1021
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The most recent developments in mammalian cell inducible expression systems have involved the use of bacterial gene control elements and viral transactivator proteins. The combination of hybrid viral transactivator and bacterial repressor proteins, and simple chemical inducers can provide induction
## Abstract ## Background Supercoiled topology of transfected plasmid DNA (pDNA) is critical for transgene expression in mammalian cells. In the present study, we analysed transgene expression of transfected supercoiled pDNA concatemers. ## Methods Jurkat T cells were transfected with a supercoi
We read with interest the article by Maucksch et al. [1], who analysed transgene expression of transfected supercoiled 4.7 kb monomeric and 9.4 kb dimeric plasmid concatemer in Jurkat T cells using electroporation. The authors reported that the relative number of electrotransferred gene copies per n