𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Pharmacological-based translational induction of transgene expression in mammalian cells

✍ Scribed by Boutonnet, Christel; Boijoux, Olivier; Bernat, Sandra; Kharrat, Abdelhakkim; Favre, Gilles; Faye, Jean-Charles; Vagner, Stéphan

Book ID
110032522
Publisher
Nature Publishing Group
Year
2004
Tongue
English
Weight
164 KB
Volume
5
Category
Article
ISSN
1469-221X

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Transgene expression of transfected supe
Transgene expression of transfected supercoiled plasmid DNA concatemers in mammalian cells
✍ Christof Maucksch; Alexander Bohla; Florian Hoffmann; Martin Schleef; Manish Kum 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 271 KB

## Abstract ## Background Supercoiled topology of transfected plasmid DNA (pDNA) is critical for transgene expression in mammalian cells. In the present study, we analysed transgene expression of transfected supercoiled pDNA concatemers. ## Methods Jurkat T cells were transfected with a supercoi

Transgene expression of transfected supe
Transgene expression of transfected supercoiled plasmid DNA concatemers in mammalian cells
✍ Jean-Michel Escoffre; Elisabeth Bellard; Muriel Golzio; Justin Teissié; Marie-Pi 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 130 KB

We read with interest the article by Maucksch et al. [1], who analysed transgene expression of transfected supercoiled 4.7 kb monomeric and 9.4 kb dimeric plasmid concatemer in Jurkat T cells using electroporation. The authors reported that the relative number of electrotransferred gene copies per n