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Gas chromatography-mass spectrometry analysis of tert.-butyldimethylsilyl derivatives of 2-acetylaminofluorene and metabolites in isolated rat hepatocytes

✍ Scribed by Miguel A. Diez Ibañez; Martine Chessebeuf-Padieu; Patrice Nordmann; Prudent Padieu

Publisher
Springer
Year
1987
Tongue
English
Weight
762 KB
Volume
3
Category
Article
ISSN
0742-2091

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✦ Synopsis

A new technique for the conversion of 2-acetylaminofluorene and several ring-hydroxylated metabolites to mono-and di-tert.butyldimethylsilyl derivatives was developed to permit their analysis by gas chromatography-mass spectrometry in order to quantify the metabolism of 2-acetylaminofluorene incubated in freshly isolated rat hepatocytes. This new gas chromatography-mass spectrometry method allowed the separation, identification and quantitation of seven known metabolites comprising five arylhydroxylated compounds, 2-aminofluorene and N-hydroxy-2-acetylaminofluorene.

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✍ Carina Norsten-Höög; Tomas Cronholm 📂 Article 📅 1990 🏛 Elsevier Science 🌐 English ⚖ 826 KB

A method for analysis of aliphatic aldehydes in biological samples is described. Cyclohexanone is added as internal standard and the samples are treated with hydroxylamine and perchloric acid. The oximes are extracted and converted to the oxime-tert-butyldimethylsilyl derivatives, which are quantita