Gas chromatographic determination of urinary methylmalonic acid
โ Scribed by Norman E. Hoffman; Joseph J. Barboriak
- Publisher
- Elsevier Science
- Year
- 1967
- Tongue
- English
- Weight
- 458 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0003-2697
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๐ SIMILAR VOLUMES
Thymol in biological samples is analyzed by gas chromatography utilizing a 5% OV-25 column, a flame ionization detector, and eugenol as an internal standard. Samples are extracted with diethyl ether and analyzed without derivatization. The lower limit of detection of thymol in a sample is about 0.01
A method for routine small-volume solvent extraction, derivitization and gasliquid chromatographic analysis of methylmalonic and succinic acids is described. The procedure allows for quantitative determination of mass and radioactivity of these acids and can be applied to metabolic studies of the ge